Macromolecular X-Ray Crystallography Core Protocols and Policies

Simple 0.1% protein labeling protocol

1. Prepare 5 mM stock solution of a succinimidyl ester dye in DMSO
2. Add appropriate amount of dye to protein solution for 0.1 % labeling of lysine residues assuming a 1:1 stochiometric labeling efficiency to amine residues
3. Wait 5 minutes for 90% dye binding

* There is no need for purification and samples are still fluorescent after 120 days